How to use an All-In-One Mushroom Grow Bag (CVG)

In this guide, you will learn how to successfully grow mushrooms using our All-In-One mushroom grow bag. This particular bag is best used with Psilocybe Cubensis varieties. We sell a sterilized gourmet all-in-one bag here.

As a side note, if the liquid culture syringe/spore syringe you are using is contaminated, the all-in-one bag will not work. Please ensure your genetics comes from a reliable supplier and have been tested.

Clean Protocol

Keeping your workspace, spawns, substrate, and cultures clean and sterile is essential to successful mushroom cultivation. Each step of the process takes a significant amount of time, and the last thing you want is for you to introduce competing bacteria to your developing mycelium, resulting in a contaminated grow.

Take the time to clean all of your tools, yourself, workspace, and supplies with isopropyl alcohol.

Cleaning supplies for mycology and wiping down your workspace — Clean, clean, clean, and clean again, with every step, clean everything.

Inoculating Your All-In-One CVG Mushroom Grow Bag

Ideally, you will want to perform these tasks in front of a flow hood or within a still-air box. At the very least, in a low-airflow room that has been cleaned with any airflow devices turned off and the door closed. This helps reduce contamination risk as the sterile mediums will be exposed to the open air while you’re performing the mixing.

Using an All in one grow bag - step 1 cleaning injection port — Clean the injection port, syringe, and hands with isopropyl alcohol before inoculation.

Using an All in one grow bag - step 2 injecting spores or liquid culture

Inject spores/liquid culture solution through the injection port.

Spores: Inject the desired amount of spores into one area. Spores germinate better in these type of bags when they are clumped together.
Liquid culture: Spread the injection as evenly as possible throughout the bag. Liquid culture will colonize quicker this way.

Tip: Wipe the injection port again after inoculation and cover it with a piece of micropore tape for extra protection against contamination.

Colonization

After injection, its time to place your bag in a warm dark place for roughly 30 days.

Avoid touching the bag too much
Check the bag once weekly
Keep in the dark & between 20C & 25C
Once the bag reaches 30% colonization, its time to break it up.

All in one grow bag mycelium colonization 10 days - Flatland Fungi — Growth after 10 days

All in one grow bag mycelium colonization 30 days - Flatland Fungi — Growth after 30 days. Its ready to be mixed.

Break & Shake

Once 30% of the bag has been colonized by mycelium its time to mix it into the rest of the bag. Colonization time will depend on the quality of your genetics (spores/LC) and the environment your keeping the bag at.

Mix the bag thoroughly, and then return it to a neat and tidy state so that the mycelium can colonize the entire bag.

A thorough mixing session takes 5 minutes per bag. From the outside of the bag, gently massage and shake the colonized mycelium into the rest of the bag to promote an even healthy fruiting colonization.

Note: Be careful with the bag, if you’re too rough with it, it may rupture creating a hole and contaminating your bag.

Fruiting Colonization

Place the bag back into the same previous colonization environment. Let it colonize for another 1 to 3 weeks (technically this phase is called the incubation phase).

Incubation times will very depending on your genetics and environment.

When the bag is ready to fruit it will look something like the picture below.

Inducing Fruiting in Your All-In-One Mushroom Grow Bag

To promote fruiting within your bag, three conditions need to change:

Increased humidity
Increased fresh air exchange
Light exposure

Increase Humidity

Mist the sides of your bag with a water spray bottle set to “fine mist setting.” You should always see condensation on the side of your bag during fruiting. However, avoid water pooling on the surface of your substrate.

Increase Fresh Air Exchange

During fruiting, mushrooms need more fresh air than during previous colonization phases. The filter patch on your bag will not be enough.

Light Exposure

The bag should be exposed to 12 hours of indirect light and 12 hours of darkness. The light source can be indirect light from a window, or a light in the room where your mushrooms are.

Temperature

To fruit your mushrooms, the temperature should be between 20 °C and 25 °C, or 68°F and 77°F.

Choosing a Fruiting Method

There are a lot of different fruiting methods for bags people like to use, ultimately you will have to test what works best for you in your environment. Below I will outline a few methods and offer some tips.

Open top fruiting:

Cut the top of your bag open.
Mist as needed
Fresh air comes in through the tiny cap created by your cut
Binder clamp or chipclip the the top to avoid too much airflow (will dry your bag out)

Dome fruiting:

Cut 90% all the way around the bag 1 inch above where the substrate sits
This allows you to fold off the top (dome), mist it, then place the dome back onto the bag
Fresh air can enter the bag through your cut while keeping it nice and humid within the dome

Grow tent fruiting:

Place the bag in a growing tent with humidifier and fresh air intake
This will reduce most of your manual labor

Tub fruiting:

Place the bag in a monotub

Mushrooms fruiting in bag — Your end result should look something like this.

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